Liver Receptor Homolog-1 (LRH-1; NR5A2)

Nuclear receptor subfamily 5, group A, member 2; Binds to the sequence element 5′-AACGACCGACCTTGAG-3′ of the enhancer II of hepatitis B virus genes, a critical cis-element of their expression and regulation. May be responsable for the liver-specific activity of enhancer II, probably in combination with other hepatocyte transcription factors. Key regulator of cholesterol 7-alpha-hydroxylase gene (CYP7A) expression in liver. May also contribute to the regulation of pancreas-specific genes and play important roles in embryonic development (541 aa) (Homo sapiens)

(From RefSeq)

NRR Pathway

lrh-1-pathway

Liver receptor homolog-1 Structure

mmdbimage-21_med

(From Structure)

Link

 

(From Aceview)

There are 65 articles specifically referring to this gene in PubMed. Functionally, the gene has been proposed to participate in  pathway (Maturity onset diabetes of the young) and processes (bile acid metabolic process, cholesterol homeostasis, positive regulation of transcription from RNA polymerase II promoter, regulation of cell proliferation, regulation of transcription, DNA-dependent). Proteins are expected to have molecular functions (lipid binding, metal ion binding, steroid hormone receptor activity, transcription activator activity, zinc ion binding) and to localize in nucleus. Putative  protein interactors have been described (ALB, CTNNB1, EDF1, NCOA3, NPPA, NR0B2, NRIP1, PNRC1, PNRC2, POLR2DANDRPS26P19ANDWDR33, PROX1, SMARCD3). 

(From HuGENavigator)

  • Pancreatic Neoplasms
  • Diabetes Mellitus, Type 2
  • Cardiovascular Diseases
  • Diabetes Complications
  • Adenocarcinoma
  • Carcinoma 
  • Irritable Bowel Syndrome 
  • Obesity 
  • Osteoporosis 
  • Overweight 
  • Diarrhea 
  • Edema 
  • Tobacco Use Disorder 

Assay Kits and Services are available from INDIGO Biosciences.

Kits are offered in different assay formats to accommodate researchers’ needs: 3x 32 and 1x 96 assay formats for screening small numbers of test compounds, as well as custom bulk reagents for HTS applications. Assay systems are all inclusive, providing reporter cells, optimized growth media, media for diluting test compounds, a positive-control agonist, luciferase detection reagent, a white assay plate, a detailed protocol, and a protocol quick guide. All kits are shipped on dry ice.

LRH-1 Reporter Cells are prepared using INDIGO’s proprietary CryoMite™ process. This cryo-preservation method yields high cell viability post-thaw, and provides the convenience of immediately dispensing healthy, division-competent reporter cells into assay plates. There is no need for intermediate spin-and-wash steps, viability determinations, or cell titer adjustments.

The principle application of this assay product is in the screening of test samples to quantify functional activities, either agonist or antagonist, that they may exert against the human liver receptor homolog-1. This kit product is an all-inclusive assay system that includes, in addition to LRH-1 Reporter Cells, two optimized media for use during cell culture and (optionally) in diluting the test samples, a reference agonist, Luciferase Detection Reagent, a cell culture-ready assay plate, and a detailed protocol.

NR5A2; B1F; CPF; FTF; B1F2; LRH1; hB1F; LRH-1; FTZ-F1; hB1F-2 or FTZ-F1beta; LOC2494

(From Aceview)

he gene contains 14 distinct gt-ag introns. Transcription produces  9 different mRNAs, 7 alternatively spliced variants and 2 unspliced forms. There are 4 probable alternative promotors, 3 non overlapping alternative last exons and 9 validated  alternative polyadenylation sites (see the diagram). The mRNAs appear to differ by truncation of the 5′ end, truncation of the 3′ end, presence or absence of 4 cassette exons, overlapping exons with different boundaries. Note that mRNA .gAug10 was found in vivo, although it is a predicted target of nonsense mediated mRNA decay (NMD). Efficacy of translation may be reduced by the presence of a shorter translated product (uORF) initiating at an AUG upstream of the main open reading frame (in variant dAug10). 

image-20_med

(From STRINGS)

Putative protein interactors have been described (ALB, CTNNB1, EDF1, NCOA3, NPPA, NR0B2, NRIP1, PNRC1, PNRC2, POLR2DANDRPS26P19ANDWDR33, PROX1, SMARCD3). 

(From KEGG)

None noted

(From BioGPS)

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