RAR-related Orphan Receptor Alpha (RORα; NR1F1)

The protein encoded by this gene is a member of the NR1 subfamily of nuclear hormone receptors. It can bind as a monomer or as a homodimer to hormone response elements upstream of several genes to enhance the expression of those genes. The encoded protein has been shown to interact with NM23-2, a nucleoside diphosphate kinase involved in organogenesis and differentiation, as well as with NM23-1, the product of a tumor metastasis suppressor candidate gene. Also, it has been shown to aid in the transcriptional regulation of some genes involved in circadian rhythm. Four transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Feb 2014]

NRR Pathway

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RAR-related Orphan Receptor Alpha Structure

(From Structure)

Link

(From Aceview)

 There are 38 articles specifically referring to this gene in PubMed. Functionally, the gene has been tested for association to diseases (Atherosclerosis; Breast Neoplasms; Leukemia, Promyelocytic, Acute; Obesity, Morbid; Translocation, Genetic), proposed to participate in pathway (Circadian rhythm – mammal) and processes (cerebellar Purkinje cell differentiation, cGMP metabolic process, nitric oxide biosynthetic process, regulation of macrophage activation, regulation of transcription, DNA-dependent). Proteins are expected to have molecular functions (metal ion binding, sequence-specific DNA binding, steroid hormone receptor activity, zinc ion binding) and to localize in nucleus. Putative protein interactors have been described (COPS5ANDLRRC67, EP300, LMO3, MED1, MYOD1, NCOA2, NME1-NME2, NR0B1, PNRC1, PNRC2, PROX1, PSMC5, RORA, SMARCD3, ZXDC). 

(From HuGENavigator)

  • Cardiovascular Diseases 
  • Asthma 
  • Diabetes Mellitus, Type 2 
  • Psychiatric Status Rating Scales 
  • Bipolar Disorder 
  • Depressive Disorder, Major 
  • Anxiety Disorders 
  • Breast Neoplasms 
  • Choroidal Neovascularization 
  • Chronobiology Disorders 
  • Depressive Disorder 
  • Geographic Atrophy 
  • Hypersensitivity 
  • Hypersensitivity, Immediate 
  • Macular Degeneration 
  • Mental Disorders 
  • Mood Disorders 
  • Nasal Polyps 
  • Obesity 
  • Sleep Disorders 
  • Stress, Psychological 
  • Tobacco Use Disorder 
  • Vasculitis 
  • Wet Macular Degeneration 
  • Disease Models, Animal 
  • Edema 

Assay Kits and Services are available from INDIGO Biosciences.

Kits are offered in different assay formats to accommodate researchers’ needs: 3x 32 and 1x 96 assay formats for screening small numbers of test compounds, as well as custom bulk reagents for HTS applications. Assay systems are all inclusive, providing reporter cells, optimized growth media, media for diluting test compounds, a positive-control agonist, luciferase detection reagent, a white assay plate, a detailed protocol, and a protocol quick guide. All kits are shipped on dry ice.

RORα Reporter Cells are prepared using INDIGO’s proprietary CryoMite™ process. This cryo-preservation method yields high cell viability post-thaw, and provides the convenience of immediately dispensing healthy, division-competent reporter cells into assay plates. There is no need for intermediate spin-and-wash steps, viability determinations, or cell titer adjustments.

The principle application of this assay product is in the screening of test samples to quantify functional activities, either agonist or antagonist, that they may exert against the human RAR-related orphan receptor. This kit product is an all-inclusive assay system that includes, in addition to RORα Reporter Cells, two optimized media for use during cell culture and (optionally) in diluting the test samples, a reference agonist, Luciferase Detection Reagent, a cell culture-ready assay plate, and a detailed protocol.

ROR1; ROR2; ROR3; RZRA; NR1F1; RZR-ALPHA

(From Aceview)

The gene contains 25 distinct gt-ag introns. Transcription produces 17 different mRNAs, 13 alternatively spliced variants and 4 unspliced forms. There are 6 probablealternative promotors, 6 non overlapping alternative last exons and 5 validated alternative polyadenylation sites (see the diagram). The mRNAs appear to differ by truncation of the 5′ end, truncation of the 3′ end, presence or absence of 6 cassette exons, overlapping exons with different boundaries. 2746 bp of this gene are antisense to spliced gene zawpee, raising the possibility of regulated alternate expression. Efficacy of translation may be reduced by the presence of a shorter translated product (uORF) initiating at an AUG upstream of the main open reading frame (in variant aAug10, bAug10, fAug10). 

image-10_med

(From STRINGS)

COPS5ANDLRRC67, EP300, LMO3, MED1, MYOD1, NCOA2, NME1-NME2, NR0B1, PNRC1, PNRC2, PROX1, PSMC5, RORA, SMARCD3, ZXDC

(From KEGG)

None noted

(From BioGPS)

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