RAR-related Orphan Receptor Gamma (RORɣ; NR1F3)

The protein encoded by this gene is a DNA-binding transcription factor and is a member of the NR1 subfamily of nuclear hormone receptors. The specific functions of this protein are not known; however, studies of a similar gene in mice have shown that this gene may be essential for lymphoid organogenesis and may play an important regulatory role in thymopoiesis. In addition, studies in mice suggest that the protein encoded by this gene may inhibit the expression of Fas ligand and IL2. Two transcript variants encoding different isoforms have been found for this gene.

[provided by RefSeq, Jul 2008]

NRR Pathway

pasted-file

 

RAR-related Orphan Receptor Gamma Structures

(From Structure)

Link

(From Aceview)

There are 29 articles specifically referring to this gene in PubMed. Functionally, the gene has been tested for association to a disease (Diabetes mellitus, type 2), proposed to participate in pathway (Circadian rhythm – mammal) and a process (regulation of transcription, DNA-dependent). Proteins are expected to have molecular functions (DNA binding, ligand-dependent nuclear receptor activity, metal ion binding, sequence-specific DNA binding and 3 others) and to localize in nucleus. Putative protein interactors have been described (CHD4, EIF4EBP1, NCOA6). 

(From HuGENavigator)

  • Lymphedema 
  • Tobacco Use Disorder 
  • Breast Neoplasms 
  • Carcinoma, Ductal, Breast 
  • Carcinoma, Lobular 
  • Celiac Disease 
  • Diabetes Mellitus, Type 2 
  • Diabetes, Gestational

Assay Kits and Services are available from INDIGO Biosciences.

Kits are offered in different assay formats to accommodate researchers’ needs: 3x 32 and 1x 96 assay formats for screening small numbers of test compounds, as well as custom bulk reagents for HTS applications. Assay systems are all inclusive, providing reporter cells, optimized growth media, media for diluting test compounds, a positive-control agonist, luciferase detection reagent, a white assay plate, a detailed protocol, and a protocol quick guide. All kits are shipped on dry ice.

RORɣ Reporter Cells are prepared using INDIGO’s proprietary CryoMite™ process. This cryo-preservation method yields high cell viability post-thaw, and provides the convenience of immediately dispensing healthy, division-competent reporter cells into assay plates. There is no need for intermediate spin-and-wash steps, viability determinations, or cell titer adjustments.

The principle application of this assay product is in the screening of test samples to quantify functional activities, either agonist or antagonist, that they may exert against the human RAR-related orphan receptor. This kit product is an all-inclusive assay system that includes, in addition to RORɣ Reporter Cells, two optimized media for use during cell culture and (optionally) in diluting the test samples, a reference agonist, Luciferase Detection Reagent, a cell culture-ready assay plate, and a detailed protocol.

TOR; RORG; RZRG; NR1F3; RZR-GAMMA

(From Aceview)

The gene contains 16 distinct introns (15 gt-ag, 1 gc-ag). Transcription produces 8 alternatively spliced mRNAs. There are 5 probable alternative promotors, 3 non overlapping alternative last exons and 6 validatedalternative polyadenylation sites (see the diagram). The mRNAs appear to differ by truncation of the 5′ end, truncation of the 3′ end, presence or absence of a cassette exon, overlapping exons with different boundaries, splicing versus retention of 6 introns. 

Gene RORC

(From STRINGS)

CHD4, EIF4EBP1, NCOA6

(From KEGG)

None noted

(From BioGPS)

206419_at_med