Thyroid Hormone Receptor Alpha (TRα; NR1A1)

(From NCBI)

The protein encoded by this gene is a nuclear hormone receptor for triiodothyronine. It is one of the several receptors for thyroid hormone, and has been shown to mediate the biological activities of thyroid hormone. Knockout studies in mice suggest that the different receptors, while having certain extent of redundancy, may mediate different functions of thyroid hormone. Alternatively spliced transcript variants encoding distinct isoforms have been reported.

Thyroid Hormone Receptor Alpha Structure

From Structure)

Link

(From Aceview)

There are 109 articles specifically referring to this gene in PubMed. Functionally, the gene has been tested for association to diseases (Adenocarcinoma, Follicular; Breast Neoplasms; Carcinoma; Cell Transformation, Neoplastic; Chromosome Aberrations; Chromosome Disorders; Deafness; Fetal Death; Liver Neoplasms; obesity and 3 others), proposed to participate in pathways (Map Kinase Inactivation of SMRT Corepressor, Neuroactive ligand-receptor interaction) and processes (adrenal gland development, brain development, cartilage condensation, cytoplasmic sequestering of transcription factor, digestive tract development and 22 others). Proteins are expected to have molecular functions (metal ion binding, protein heterodimerization activity, protein homodimerization activity, steroid hormone receptor activity and 3 others) and to localize in various compartments (nucleus, cytoplasm, mitochondrion). Putative protein interactors have been described (AKAP13, BTG1, CCND1, CDK7, CDK8, COPS2, CREB1, CREBBP, CUL1, DAP3 and 40 others). 

(From HuGENavigator)

  • Alzheimer Disease  
  • Thyroid Neoplasms  
  • Osteoporosis
  • Cardiovascular Diseases  
  • Coronary Disease  
  • Diabetes Complications  
  • Fractures, Bone  
  • Hypertension
  • Metabolic Syndrome X  
  • Neoplasms  
  • Osteoarthritis  

Assay Kits and Services are available from INDIGO Biosciences.

Kits are offered in different assay formats to accommodate researchers’ needs: 3x 32, 1x 96, and 1x 384 assay formats for screening small numbers of test compounds, as well as custom bulk reagents for HTS applications. Assay systems are all inclusive, providing reporter cells, optimized growth media, media for diluting test compounds, a positive-control agonist, luciferase detection reagent, a white assay plate, a detailed protocol, and a protocol quick guide. All kits are shipped on dry ice.

TRα Reporter Cells are prepared using INDIGO’s proprietary CryoMite™ process. This cryo-preservation method yields high cell viability post-thaw, and provides the convenience of immediately dispensing healthy, division-competent reporter cells into assay plates. There is no need for intermediate spin-and-wash steps, viability determinations, or cell titer adjustments.

The principle application of this assay product is in the screening of test samples to quantify functional activities, either agonist or antagonist, that they may exert against the human thyroid hormone receptor. This kit product is an all-inclusive assay system that includes, in addition to TRα Reporter Cells, two optimized media for use during cell culture and (optionally) in diluting the test samples, a reference agonist, Luciferase Detection Reagent, a cell culture-ready assay plate, and a detailed protocol.

AR7; EAR-7.1/EAR-7.2; ERBA; NR1A1; THRA3

(From Aceview)

The gene Contains 21 distinct introns (19 gt-ag, 2 gc-ag). Transcription produces 16 different mRNAs, 13 alternatively spliced variants and 3 unspliced forms. There are 8 probable alternative promotors, 5 non overlapping alternative last exons and 5 validated alternative polyadenylation sites (see the diagram). The mRNAs appear to differ by truncation of the 5′ end, truncation of the 3′ end, presence or absence of 6 cassette exons, overlapping exons with different boundaries, splicing versus retention of 4 introns. 1084 bp of this gene are antisense to spliced gene NR1D1, raising the possibility of regulated alternate expression. Efficacy of translation may be reduced by the presence of a shorter translated product (uORF) initiating at an AUG upstream of the main open reading frame (in variant cAug10, fAug10). 

image-2_med

(From STRINGS)

NCOR1, RXRA, NCOR2, NCOA1, Rxr, MED1 (includes EG:19014), NCOA2, NRIP1, GTF2B, NCOA6, GRIP1, RXRB, HR, ncor, COPS2

(From KEGG)

  • 3,5-diiodothyropropionic acid
  • amiodarone
  • L-triiodothyronine
  • levothyroxine

(From BioGPS)

1316_at_med